This paper puts forward a construction method based on ontology for the Pearl River Basin fish production, to facilitate the domain knowledge analysis and information retrieval. By converting the concepts and terms in domain ordinally, the fish production ontology was constructed with the definition of classes, properties, instances, and relationships. The developed ontology model of the fish production knowledge is proposed and applied in the system of fish diseases diagnosis primarily. The research lays the semantic foundation for the further efficient knowledge management and practical application.