Detection of ultra trace amount gossypol is reported. It is based on luminol- hexacyanoferrate(III) chemiluminescence reaction with gossypol as a sensitizer. The detection limit (3σ)of gossypol reaches 5.21×10-16 molL.
Er Bao LIU Min ZHANG Bing Chun XUE Peng Xiang ZHAO Yin LIU
A method of on\|line ultrasensitive chemiluminescence detection with capillary electrophoresis for Co(Ⅱ) is reported. Using our newly developed capillary electrophoresis with chemiluminescence detection system and novel mixing mode of the reagents, the effects of field\|amplified sample injection on detection limits of metal ions were studied in detail. The sub\|fmol/L level(0.13 fmol/L, 1.6 ymol) detection of cobalt ions in ultradilute solution was performed. The separation of fmol/L level Co(Ⅱ) and trace amounts of Ni(Ⅱ) was performed successfully.
This work reports the single-molecule detection of gossypol by flow injection analysis with chemiluminescence method. The method is based on the reaction of luminol with ferricyanid in sodium hydroxide medium sensitized by gossypol. Under the optimum conditions, the CL intensity is proportional to the concentration of gossypol over the range of 1.11×10-17-2.78×10-16 mol/L in acid solution and 8.00×10-11-7.39×10-8 mol/L in neutral solution with correlation coefficients 0.9983 and 0.9905, respectively. The detection limits is 1.60×10-18 mol/L (S/N=3). The proposed method has been applied for the determination of the gossypol in cottonseeds and pharmaceutical formulations with satisfactory results.
