In January 2016, Kunming underwem 52 hours of rain and snow weather below 0℃. in order to identify the cold tolerance of different wild sugarcane germplasm resources in low temperature and snow disasters, cold injuries of 38 shares of wild sugareane germplasm resources, which were oollected during 2012 -2013 and preserved in the germplasm resource nursery of Sugarcane Research Institute, Yumlan Agricultural University, were surveyed to evaluate the cold tolerance based on sugarcanc cold tolerance index (CTI) = 0.5 x shoot tip growing point injury index + O. 3 x node injury index + 0.2X internode injury in- dex. The results showed that cold tolerance indexes of four wild sugarcane varieties demonstrated a descending order of Erianthus arundinaceum Retz. (0.7), Eri- aTrthus rockii Keng (0.5) , Sacchartun sporrtanettm L. (0.3) , Erianlhus fulvus Ne^s. (0). Greater cold tolerance indexes indicate poorer cold tolerance. Thus, Erianthu, arundinaceum had the poorest cold tolerance, while Erianthus fulvus exhibited the strongest cold tolerance. In addition, there were significant differences in cold tolerance among diffcrent Saccharum spontaneum clones. The 23 Saccharum spontaneum clones could be divided into four grades, including 1 clone with the poorest cold tolerance that accounted for 4.3%, 5 clones with poor cold tolerance that accounted for 21.7%, 14 clones with strong cold tolerance that accounted tot 60.9%, and 3 clones with the strongest cold tolerance that accounted for 13.0% (2012 -23, 2012 -32, 2012 -37). Especially, 2012 -23 exhibited extremely strong cold tolerance. This study provided the scientific basis for screening of cold-tolerant wild sugarcane germplasm resources and selection of cold-toleranl parents.