Objective：To investigate the effects of Xiezhuo Chubi Decoction（泄浊除痹方,XZCBD） on the microRNA expression patterns of kidney in mice with hyperuricemia.Methods：Sixty Kunming male mice were randomly divided into the high-,medium-,and low-dose XZCBD groups,benzbromarone group,model group, and control group.Except the control group,all mice were established with yeast method combined with uricase inhibition method to build hyperuricemia model,and the corresponding drugs（37.5 g/kg,18.75 g/kg,9.375 g/kg, and 0.02 g/kg per day） were administrated on the 7th day.On the 22nd day,the blood uric acid concentration was detected,and microRNA with obvious changes in kidney was screened with qRT-PCR.Results：The uric acid in the model group was higher than that in the control group,and the levels of the uric acid were reduced after being treated with XZCBD; the differences among groups were significant（P〈0.05）.Compared with the control group,32 kinds of microRNA expression changes were detected on the 15th day after being treated with high-dose XZCBD by microRNA expression profile screening.Among them,miR-34a could inhibit the expression of human urate anion exthanger 1,and miR-146a might have inhibited the inflammatory reaction.Conclusion： XZCBD could significantly reduce the serum uric acid level; its effect on hyperuricemia might be through affecting microRNA expressions.
Objective： To explore the effect of Compound Tufuling Granules （复方土茯苓颗粒, CTG） on regulating glucose transporter 9 （GLUT9） expression in the kidney to influence the uric acid excretion by the kidney and serum uric acid （SUA） level in hyperuricemia mice. Methods： Sixty Kunming male mice were randomly divided into the control group, model group, benzbromarone group, and CTG high-, middle- and low- dose groups. The yeast extract and uricase inhibition method were used to build hyperuricemia model, and the corresponding drugs were administrated on the 7th day. On the 21st day the 24-h urine was collected, on the 22rid day the blood was collected, the SUA level was detected by uricase colorimetry, and the mRNA and protein expressions of GLUT9 were detected by quantitative real-time polymerase chain reaction and Western blot, respectively. Results： Compared with the model group, the levels of SUA and the mRNA and protein expressions of GLUT9 were significantly decreased, and the fraction excretion of uric acid （FEUA） was significantly increased in the CTG groups and benzbromarone group （all P〈0.05）. There was no significant difference in the above indicators between the CTG high-dose group and benzbromarone group （P〉0.05）. SUA is positively related to the GLUT9 mRNA and protein expressions in the kidney （P〈0.05 or P〈0.01）. Conclusions： CTG can significantly reduce the SUA and increase the FEUA. In addition, CTG can effectively inhibit the mRNA and protein expressions of GLUT9 in the kidney of hyperuricemia mice to inhibit the uric acid re-absorption, promote uric acid excretion and reduce SUA.
