自20世纪初以来,中医变革经历的"中西汇通"或"中西医结合"、"中医科学化"和"中医现代化"等运动的洗礼。然而,尚未发现一条成功的路线。从理论结构和逻辑上分析,笔者认为,中医本质上是一种基于"自然自愈力the healing power of nature"的唯象医学体系。在疾病治疗策略的六种层次中,中医中药主要在高层次(3~6)阶段发挥治疗作用。中医的本质决定了中医现代化不可能是用现代科学的方法对中医进行解构和解析,而是应当采取一种在中医先验"假设"的指导下、从西医出发"建构"创新的医学体系。目前,研究建立科学性的脏腑关系、基于经络调节的新神经免疫学和基于细胞信号传导的中药活性评价系统是可为之工作。本文提供了一个能够在坚持中医特色的基础上推进中医的现代化成功的路线图。
The tight junction disorder plays an important role in the pathological process of many chronic diseases, and is becoming a major concern for the clinical application of metal drugs, i.e. anti-diabetic vanadium compounds. The development of novel tight junction protecting agents has thus been a major research focus. Since oxidative stress is the primary cause for vanadium toxicity, the present work tested the protective effects of zinc gluconate （Zn2＋） alone and when combined with vitamin C （VC） on the vanadium compound （VO（acac）z.）-mediated paracellular leakage of MDCK cells. The experimental results showed that VO（acac）2_ treatment significantly increased the paracellular permeability of MDCK monolayer. Zn2＋ alone showed no protective effects and VC ameliorated tight junction leakage of MDCK cells when given in the basal chamber. Interestingly, unilateral treatment with the combination of Zn2＋ and VC effectively prevented the increase of paracellular permeability. In addition, the combination of zinc and VC down-regulated the levels of reactive oxygen species in both the control and VO（acac）2-treated MDCK cells and caused the elevation of intracellular Ca2＋; both effects were beneficial for the maintenance of integrity of intercellular tight junction. Our results provided a simple but very effective method of preventing the metal toxicity for clinical aoNication of anti-diabetic vanadium compounds.
