为了解决生物科学方面的研究人员在使用生物研究软件工具中存在的"不知道、选不出、难学习和用不起"等问题,同时也为了能为生物类院校的师生提供教学辅助,利用网格技术在高性能服务器上,建立了一款服务于生物研究和教学的网上生物科研服务平台(Biological study service platform,BSP)。该平台包含"工具大全、工具论坛和web工作台"等三部分,拟分别从"找,学,用"三个方面彻底解决生物研究人员在使用生物研究软件工具中存在的问题,同时也为生物类的教师和学生提供了教与学平台。访问地址如下:http://218.57.145.30:9999/biosp/。此外,该研究小组还将不断地更新和改进该平台,开发更多的自主应用软件,更好地服务生物科研工作。
The nucleotide sequence of the melanocortin-l-receptor （MC1R） gene was studied with the help of the polymerase chain reaction （PCR）, in which the protein structure in Chinese Holstein was predicted, and the molecular mechanism of the red coat color was investigated. Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） was performed to genotype the individuals. The bioinformatics and biotechnology softwares were used to predict the secondary structure of MC1R. The results showed that the EE genotype was the dominant genotype in Chinese Holstein Black and White herd, whereas, it was ee in Chinese Holstein Red and White herd. The secondary structure of the mutational MC1R protein was changed and the deletion mutation caused an earlier termination in translation, which led to the formation of the red coat color. The allele E was mainly associated with the black coat color, whereas, e was associated with red.
LI Qiu-lingLI Jian-binZHANG Zheng-fengWANG Hong-meiWANG Chang-faGAO Yun-dongHou Ming-haiZHONG Ji-feng
