[Objective] This study aimed to investigate a reliable method for DNA ex- traction from Wusuli raccoon dog＇s hair. [Method] Several DNA extraction methods were used to extract DNA from Wusuli raccoon dog hair, including Chelex-100 method, PCR buffer method, organic phenol-chloroform method and centrifugal col- umn type kit method. The extracted DNA was analyzed by using PCR amplification and electrophoresis to compare these four DNA extraction methods. [Result] Accord- ing to the results of spectrophotometer detection and gel electrophoresis, nucleic acid extracted by Chetex-100 method had proteins and other impurities; nucleic acid ex- tracted by PCR buffer method was low in concentration; however, DNA extracted by organic phenol-chloroform method and centrifugal column type kit was high in con- centration with no impurity band. [Conclusion] This study had laid the strong founda- tion of scientific theory to further explore the efficient and simple method for extracting DNA from Wusuli raccoon dog hair follicle.
[ Objective] This study aimed to lay theoretical foundation for establishing the database of biological characteristics of raccoon dogs with different coat colors. [Method] Reddish-brown ussuri raccoon dogs, wild ussuri raccoon dogs and Jilln white raccoon dogs were used as experimental materials in this study. Chromosome specimens were prepared by peripheral blood lymphocyte culture, to analyze chromosome number of ussuri raccoon dogs with different coat colors. [ Result] Frequencies of cells with diploid chromosome number of 56 in reddish-brown ussuri raccoon dogs and wild ussuri raccoon dogs were 86% and 83% re- spectively, indicating that the chromosome number of reddish-brown ussuri raccoon dogs and wild ussuri raccoon dogs was 56. The frequency of cells with diploid chromosome number of 56 in Jilin white raccoom dogs was 70%, which was lower than that of other two varieties, while the frequency of cells with 2n = 57 was high- er than that of other two varieties. [Conclusion] The chromosome number was 56 for reddish-brown ussuri raccoon dogs and wild ussuri raccoon dogs, and it was 56 or 57 for Jllin white raccoon dogs, which represented pelymorphism.
