By analyzing the principle of process variations, a lightweight Physical Unclonable Function (PUF) circuit based on selectable cross-coupled inverters is proposed in this paper. Firstly, selectable cross-coupled inverters are chosen for two delay paths. Simultaneously, the circuit takes challenge signal to control each delay path. The PUF cell circuit is implemented in Semiconductor Manufacturing International Corporation (SMIC) 65 nm CMOS technology and the layout area is 2.94μm × 1.68μm. Then the 64-bit PUF circuit is achieved with the cascade connection of cell circuits. The simulation results show that the randomness is 49.4% and the reliability is 96.5%. Compared to the other works, this PUF circuit improves the encrypt performance and greatly reduces the area.