The effect of cold stress on the gene expression of cold-inducible RNA-binding protein （CIRP） in the intravital animals has not been reported till now. Compared with their organism cells, there were much more complicated regulatory mechanisms for cold stress response in the organisms. The BALB/C mice with cold treatment were used as experimental animals for this study. The cDNA of CIRP was firstly cloned from the testis tissues of the BALB/C mice treated by cold stress The results indicated that CIRP in the organisms could be induced at low temperature and may protect the organisms from the cold damage. The amino acid sequence deduced via cDNA clone was 100%, 99.4%, 95.5%, 67.4%, 76.9%, 79.1% and 58.4% identical with that of CIRP in mice, rats, human, bullfrog, Xenopus and axolotl cells, respectively. These results showed that the CIRP was highly conserved in the evolution process and may be involved in various physiological functions. Therefore, this study will establish a systematic model for experiments and provide a new foundation for exploring the molecular mechanisms of human and other animals under cold stress.
