The Erguna Fault runs along the east bank of the Erguna River in NE China and is a large-scale ductile shear zone comprising granitic mylonites. This paper reports on the geometry, kinematic indicators, and 40Ar/39 Ar biotite ages of the granitic mylonites, to constrain the structural characteristics, forming age, and tectonic attribute of the Erguna ductile shear zone. The zone strikes NE and records a top-to-the-NW sense of shear. A mylonitic foliation and stretching lineation are well developed in the mylonites, which are classified as S-L tectonites. Logarithmic flinn parameters(1.18–2.35) indicate elongate strain which approximates to plane strain. Kinematic vorticity numbers are 0.42–0.92 and 0.48–0.94, based on the polar Mohr diagram and the oblique foliation in quartz ribbons, respectively, suggesting that the ductile shear zone formed under general shear, or a combination of simple and pure shear. According to finite strain and kinematic vorticity analyses, the Erguna Fault is a lengthening-thinning ductile shear zone that formed by extension. The deformation behavior of minerals in the mylonites indicates that the fault was the site of three stages of deformation: an initial stage of middle- to deep-level, high-temperature shear, a post-stress recovery phase of high-temperature static recrystallization, and a final phase of low-temperature uplift and cooling. The 40Ar/39 Ar plateau ages of biotite from the granitic mylonites are 106.16 ± 0.79 and 111.55 ± 0.67 Ma, which constrain the timing of low-temperature uplift and cooling but are younger than the ages of metamorphic core complexes(MCCs) in the Transbaikalia-northeast Mongolia region. Using measured geological sections, microtectonics, estimates of finite strain and kinematic vorticity, and regional correlations and geochronology, we conclude that the Erguna Fault is an Early Cretaceous, NNE-trending, large-scale, sub-horizontal, and extensional ductile shear zone. It shares a similar tectonic background with the MCCs, volcanic fault basins