This study aims to save cost of sampling for estimating the area under the amlodipine plasma concentration versus time curve in 24 hours(AUC0?24 h).Limited sampling strategy(LSS) models was developed and validated by mutilple regression model within 4 or fewer amlodipine concentration values.Absolute prediction error(APE),root of mean square error(RMSE) and visual predict check were used as criterion.The results of Jackknife validation showed that fifteen(9.4%) of the 160 LSS based on regression analysis were not within an APE of 15% by using one concentration-time point.156(97.5%),159(99.4%) and 160(100%) of the 160 LSS model were capable of predicting within an APE 15% by using 2,3,4 points,separately.Limited sampling strategies have been developed and validated for estimating AUC0?24 h of amlodipine.The present study indicated that the implemention of both 5 mg and 10 mg dosage could enable accurate predictions of AUC0?24 h by the same LSS model.This study shows that 12,4,24,2 h after administration are key sampling time points.The combination of(12,4),(12,4,24) or(12,4,24,2 h) might be chosen as sampling hours for predicting AUC0-24 h in practical application according to requirement.
