Objective:To explore the effect of “Runing Ⅱ” on the tumor growth and metastasis of Human Breast Cancer Cell Line MDA MB 435 in Vivo and Vitro. Methods: Both in vitro and in Vivo experiments were conducted in the study. In Vitro, MTT method was used to detect the ability killing the tumor cells of serum with “Runing Ⅱ”. In vivo, We have observed the effect of “Runing Ⅱ” on the tumor growth and metastasis by nude mice model implanted MDA MB 435. Result: At the dosages of 32g, 16g and 8g per kilogram, “Runing Ⅱ” could kill MDA MB 435 cells 35 88%、29 84% and 8 30% respectively in vitro, the inhibitory effect of tumor growth was statistially significant, as compared with that of the normal group ( P <0 05),there were no statistital difference between the “Runing Ⅱ” group at the dosages of 48g/kg and the CTX group ( P>0 05 ). In the case of animal experiments, the Human Breast Cancer Cell Line MDA MB 435 was inoculated into the m.f.p. of nude mice, the results demonstrated that the growth suppression rate and the Lung metastasis inhibiting rate of “Runing Ⅱ” at the dosages of 48g/kg was 53 64% and 27% respectively, which were no statistial difference than that of CTX group (the growth suppression rate and the Lung metastasis inhibiting rate was 48 8% and 25 2% respectively)( P> 0 05). Conclusion: The results suggest that “Runing Ⅱ” can suppress the tumor growth and metastasis of Human Breast Cancer Cell Line MDA MB 435.
