The mitochondria of anther at pollen mother cell stage of cytoplasmic male sterile (CMS) line 3197A in Sorghum were extracted after heat shock. The number of mitochondria was measured with now cytometen and the isoenzymes in mitochondria were analyzed by PAGE combined with staining for enzyme activity. The result of quantity measurement of mitochondria showed that the number of mitochondria in CMS line 3197A increased greatly up to 4.5 folds after heat shock. The analysis of isoenzymes also demonstrated that the type of isoenzymes in CMS line was in accordance with the maintainer line. The study first showed the change of the quantity of mitochondria after heat shock and the relationship between this change and discussed the fertility in sorghum.
