您的位置: 专家智库 > >

国家自然科学基金(30770131)

作品数:10 被引量:40H指数:4
相关作者:于恒秀龚志云程祝宽刘巧泉王歆更多>>
相关机构:扬州大学中国科学院遗传与发育生物学研究所中国科学院上海生命科学研究院更多>>
发文基金:国家自然科学基金国家高技术研究发展计划教育部“新世纪优秀人才支持计划”更多>>
相关领域:农业科学生物学更多>>

文献类型

  • 9篇中文期刊文章

领域

  • 8篇农业科学
  • 3篇生物学

主题

  • 5篇水稻
  • 4篇基因
  • 3篇转基因
  • 3篇转基因水稻
  • 3篇基因水稻
  • 2篇农杆菌
  • 2篇GFP
  • 1篇稻子
  • 1篇端粒
  • 1篇端粒酶
  • 1篇新品系
  • 1篇再生植株
  • 1篇植株
  • 1篇软米
  • 1篇水稻突变体
  • 1篇突变体
  • 1篇农杆菌介导
  • 1篇糯稻
  • 1篇品系
  • 1篇中籼

机构

  • 6篇扬州大学
  • 4篇中国科学院遗...
  • 1篇中国科学院上...

作者

  • 5篇于恒秀
  • 4篇程祝宽
  • 4篇龚志云
  • 3篇刘巧泉
  • 3篇王歆
  • 3篇唐丁
  • 2篇顾铭洪
  • 2篇裔传灯
  • 2篇陆美芳
  • 1篇曾燕楠
  • 1篇王宗阳
  • 1篇蔡秀玲
  • 1篇徐丽
  • 1篇陈秀花
  • 1篇黄健

传媒

  • 2篇Rice s...
  • 1篇中国农业科学
  • 1篇科学通报
  • 1篇中国水稻科学
  • 1篇作物学报
  • 1篇扬州大学学报...
  • 1篇Scienc...
  • 1篇分子植物育种

年份

  • 3篇2009
  • 6篇2008
10 条 记 录,以下是 1-9
排序方式:
一个显性矮秆水稻突变体的获得及其遗传分析被引量:7
2008年
【目的】分析该研究组已发现的一个显性矮秆水稻突变体的遗传组成及背景。【方法】利用农杆菌介导法转化粳稻品种武香粳9号,产生T-DNA插入群体。在筛选和鉴定水稻T-DNA插入突变体的过程中,发现一个矮秆突变体。利用PCR扩增、Southern杂交等分子生物学方法对该突变体后代进行鉴定及遗传分析。【结果】突变体自交后代群体中出现矮秆和正常株高两种类型,分离比为3﹕1,符合一对显性单基因的遗传,并且矮秆性状的表现与T-DNA插入共分离。利用籼稻品种龙特甫与其纯合矮秆植株进行杂交,杂交F2代的矮秆株与正常株的比例同样呈3:1分离,符合一对显性单基因的遗传规律。利用SSR等分子标记,将该基因定位在水稻的第4染色体上。【结论】该矮秆性状由一对显性基因控制,由T-DNA插入引起,位于水稻第4染色体上,可作为进一步分离该基因的遗传材料。
王歆于恒秀唐丁黄健龚志云程祝宽
关键词:水稻显性矮秆基因T-DNA插入
无抗性选择标记转基因软米和糯稻新品系的选育及中间试验被引量:7
2009年
直链淀粉含量是影响稻米品质的最主要因素之一。为培育直链淀粉含量较低的软米和糯稻新品系,在经共转化法获得的转反义Wx基因水稻自交后代中筛选获得了不含抗性选择标记基因的纯合体。对这些纯合转基因水稻的分析表明,其未成熟种子胚乳中Wx基因表达水平和成熟种子中Wx蛋白量较未转化对照有不同程度的降低。转基因水稻成熟种子中直链淀粉含量较未转化对照有不同程度的下降,2个转基因系降低至10%左右,相当于软米的直链淀粉含量,1个转基因系降到了2%以下,形成了糯稻新品系。转基因稻米中直链淀粉含量降低后,其胶稠度相应变软,而糊化温度未发生明显改变。田间试验结果表明,转基因水稻的大部分农艺性状与受体品种武香粳9号无显著差异。因此,本研究成功培育了无抗性选择标记且淀粉品质改良的转反义Wx基因水稻新品系。
于恒秀刘巧泉徐丽陆美芳蔡秀玲龚志云裔传灯王宗阳顾铭洪
关键词:转基因水稻反义WX基因软米糯稻
不同转化方法培育无抗性选择标记转基因水稻效率的比较被引量:14
2009年
为研究不同的转化方法培育无抗性选择标记转基因水稻的效果,以4个籼粳稻品种为受体材料,比较了用农杆菌介导的双菌双载体和双T-DNA单载体两种共转化技术的共转化频率及随后获得的无抗性选择标记基因转化子的效率。结果表明,在双菌双载体介导的转化中,4个水稻品种的平均共转化频率为10.1%,其中55.6%的共转化植株自交后代中可筛选出无抗性选择标记的转基因水稻植株。在单载体双T-DNA介导的共转化中,平均共转化频率为45.0%,从其中60.0%的共转化植株自交后代中可获得无抗性选择标记的转基因植株,即双T-DNA单载体法的去选择标记的效率(27.0%)要较双菌双载体法(5.6%)高。
于恒秀陆美芳陈秀花龚志云刘巧泉顾铭洪
关键词:水稻农杆菌共转化
Generating of rice OsCENH3-GFP transgenic plants and their genetic applications
2008年
In order to investigate rice functional centromeres, OsCENH3-GFP chimeric gene was constructed and transformed into the indica rice variety, Zhongxian 3037, mediated by Agrobacturium. The integration of the exogenous genes in the transgenic plants was confirmed by PCR and Southern blotting. The transgenic plants grow normally during their whole life time, just like Zhongxian 3037. No significant defects were detected in either mitosis or meiosis of the transgenic plants. The overlapping of GFP signals and anti-CENH3 foci in both mitotic and meiotic cells from T0 and T1 generation plants indicated that GFP had been successfully fused with CENH3, so the GFP signals can well represent the CENH3 locations on each chromosome. To evaluate the applicability of the transgenic plants to other genetic studies, fluorescence in situ hybridization (FISH) using rice centromeric tandem repetitive sequence CentO as the probe was conducted on the zygotene chromosomes of pollen mother cells (PMCs). It has been revealed that the GFP signals are overlapping with CentO FISH signals, showing that CentO is one of the key elements constituting rice functional centromeres. Immunofluorescent staining using anti-α-tublin antibody and anti-PAIR2 antibody on the chromosomes during mitosis and meiosis stages of the transgenic plants further reveals that OsCENH3-GFP transgenic plants can be widely used for studying rice molecular biology, especially for tagging functional centromeres in both living cells and tissues.
YU HengXiuWANG XinGONG ZhiYunTANG DingGU MingHongCHENG ZhuKuan
关键词:着丝点稻子
Efficiencies of Generating Selectable Marker-Free Transgenic Rice with Different Transformation Methods
2009年
To study the efficiency of generating selectable marker-free (SMF) transgenic rice, two transformation methods were employed for four rice varieties (Wuxiangjing 9, Longtefu, Xieqingzao and Zhenshan 97). One method is by using a single twin T-DNA binary vector pYH592 in one Agrobacterium strain, which is composed of two separate T-DNA regions (one carrying an antisense Wx gene and the other carrying a HPTgene). The other one, named as two-strain/two-vector system, is by using two separate binary vectors in two separate Agrobacterium cultures. The results indicated that the average co-transformation frequencies of the antisense Wx gene and the HPT gene were 10.1% and 45.0%, respectively, for the four rice varieties. And the SMF transgenic plants selected from the offsprings of co-transformants were 55.6% and 60.0% in the two-strain/two-vector and twin T-DNA vector binary systems, respectively.
Yu Heng-xiu Lu Mei-fang CHEN Xiu-hua GONG Zhi-yun LIU Qiao-quan Gu Ming-hong
关键词:CO-TRANSFORMATION
Molecular-Cytological Identification and Chromosome Behavior Analysis of Telotetrasomic in Rice
2008年
From the progenies of a telotrisomic of chromosome 9 short arm of an indica rice variety, Zhongxian 3037, a phenotypical variant was selected. The variant plant had rolled leaves, dispersed plant type, as well as a low seed-setting rate. Cytological and molecular cytological investigations revealed two extra chromosomes, which were the shortest in somatic cells of the variant. Fluorescent in situ hybridization (FISH) analysis using a rice centromere specific DNA (RCS2) and a DNA sequence specific for chromosome 9 on premetaphase and pachytene chromosomes showed that these two chromosomes were the short arms of chromosome 9. That is to say, the variant was a telotetrasomic of chromosome 9. Among the 25 pachytene cells, the two telosomic chromosomes paired each other to form a bivalent and didn't pair with other normal chromosome 9 as multivalents in 96% cells. However, the bivalent was easy to disassociate in advance.
GONG Zhi-yun GAO Qing-song Yu Heng-xiu YI Chuan-deng Gu Ming-hong
关键词:RICEMEIOSIS
农杆菌介导籼稻中籼3037转化体系的优化及其应用被引量:8
2008年
以籼稻品种中籼3037为材料,研究了影响农杆菌介导转化籼稻的几个重要因素,结果表明:MSⅡ培养基作为愈伤组织的诱导培养基好于N6D2;以幼胚为外植体,愈伤组织的诱导率和转化率明显比成熟胚和幼穗高;根癌农杆菌菌株EHA105介导的基因转化率高于AGLO;当以幼胚为外植体,根癌农杆菌菌株E-HA105介导的中籼3037的转化率为18.0%,而AGLO为5.5%;EHA105菌液OD值为0.6,浸染时间14min为中籼3037合适的转化参数;在分化培养基MSR中添加20g/L的山梨醇有利于抗性愈伤组织的分化。在此基础上,采用优化的农杆菌介导法转化中籼3037及其来源的突变体A和B,获得了转化植株,PCR、Southern杂交和T1代遗传分析表明外源基因已经整合到水稻基因组中。
王歆于恒秀曾燕楠刘巧泉龚志云程祝宽
关键词:根癌农杆菌籼稻
RNA干涉水稻端粒酶再生植株的初步研究
2008年
依据水稻端粒酶基因的相关生物学信息,构建了含有水稻端粒酶序列RNA干扰结构的植物表达载体pC am 23A-1-2,并利用农杆菌介导法将目的基因导入到粳稻品种日本晴中,获得了78个独立转化子,共165棵转基因植株。通过PCR和Southern杂交分析,证明RNA干扰结构已整合进水稻基因组中;应用染色体末端限制性片段分析法,显示在转基因水稻中端粒DNA序列长度有逐代缩短的趋势,初步证明这些转基因水稻中端粒酶亚基已失活,但是T0和T1代转基因水稻植株的主要农艺性状没有发生明显变化。
王歆于恒秀唐丁裔传灯程祝宽
关键词:水稻端粒酶RNA干涉
OsCENH3-GFP融合转基因水稻的获得及其遗传应用被引量:2
2008年
通过农杆菌介导的方法,将水稻组蛋白H3与绿色荧光蛋白嵌合基因(OsCENH3-GFP)导入水稻品种中籼3037中,经PCR及Southern blot检测,证明该嵌合基因确已整合到水稻的基因组中.该转基因植株发育正常,有丝分裂及减数分裂行为均正常.对T0及T1代转基因水稻植株有丝分裂和减数分裂过程中GFP与水稻CENH3表达关系的研究结果表明,CENH3的表达部位与GFP的表达部位完全重叠,说明GFP与水稻CENH3已构成融合蛋白,并且定位于染色体的着丝粒部位.为探索该转基因植株在水稻遗传及分子生物学研究中的作用,利用花粉母细胞减数分裂偶线期染色体,借助水稻着丝粒串联重复序列CentO的FISH,发现GFP信号与CentO信号完全重叠,证明CentO序列确实为水稻不同染色体功能性着丝粒的主要成分.利用该转基因植株,分别制作根尖细胞有丝分裂染色体和花粉母细胞减数分裂染色体制片,与anti-α-tublin抗体和anti-PAIR2抗体进行荧光免疫染色反应,表明该转基因植株携带GFP标记的着丝粒,可以与其他分子生物学手段相结合,并能在活体细胞及组织内十分方便地显示每一染色体功能性着丝粒位置,为深入研究着丝粒的功能提供了宝贵的遗传材料.
王歆于恒秀龚志云唐丁顾铭洪程祝宽
关键词:水稻着丝粒GFP
共1页<1>
聚类工具0