In vitro bioassay screening systemically,we find that the crude extract of mangrove endophytic fungus Paecilomyces sp.(tree1-7)from the Taiwan Strait showed strong inhibiting to KB cells,and isolate three isomeric compounds,secalonic acid A(1),penicillixanthone A(2),paecilin A(3).Their structures are elucidated by spectral data,and the 3 is the first time to be isolated from marine fungus.The results of primary bioactivity experiments indicate that the three compounds display strong inhibiting activity to KB cell in vitro,and 1 show inhibiting to KB and KBv cells at IC50 less 1.57nmol/mL,2 at IC50 less 1.22nmol/mL and 3 at IC50 40,50nmol/mL,respectively.
Eight compounds are isolated from the ferment liquid of mangrove fungus(No.zzf33) by silica gel column chromatography.Their structures are determined to be ramlusin(1),monodictyxanthone(2),8-hydroxy-6-methyl-9-oxo-9H-xanthene-1-carboxylic acid methyl ester(3),(E)-dec-2-enedioic acid(4),succinic acid(5),urail(6),thymine(7) and nonacosanoic acid(8) on the basis of comprehensive spectroscopic analysis and chemical method.Compounds 1-3 are isolated from Penicillium sp.for the first time.Compound 8 is first found in marine fungus.In the primary bioassay,compound 2 and 3 exhibit high inhibition to hep2 and hepG2 cells lines.
Six alkaloids,2-(1H-indol-3-yl)-4H-benzo[d]oxazin-4-one(1),methyl o-(indole-3-carboxamido) benzoate(2),cyclo(Ile-Leu-Val-Va1)(3),cyclo(Phe-Gly)(4),cyclo(Leu-Leu)(5),and cyclo(Ala-Leu)(6),were isolated from the mangrove endophytic fungus ZSU-H26 from the South China Sea.Their structures were elucidated by spectral data.Indole alkaloids 1 and 2 were both isolated from microorganism for the first time.In the preliminary bioassay,1 and 2 show inhibitory activity on the hepG2 cell line with IC50 values of 20μg/mL and 35μg/mL,respectively.
