[Objective] The study aimed at assessing the ecological security of Red River basin in Guangxi. [Method] Firstly, the ecological security assessment index system of Red River basin was established based on the framework of 'pressure-state-response' model, and index information of ecological security assessment was extracted by using RS and GIS technology; afterwards, the ecological security of Red River basin was divided into five grades according to ecological security index, and the distribution and characteristics of ecological security at various levels were analyzed; finally, the measures to maintain the ecological security of Red River basin were put forward on the basis of problems in ecological security. [Result] Most areas of Red River basin in Guangxi were in generally safe state, especially Lingyun County, Fengshan County, Du'an County, Dahua County, Shanglin County, Binyang County, Guiping City, etc., and the area accounted for 74.25% of total area; next came safer state (12.74%), the regions in the two states above were the most important environmental areas of Red River basin. The ecological security problems of Red River basin were mainly related to fragile ecological environment, lagging economic development, rapid population growth, excessive development and utilization of natural resources and so forth. [Conclusion] The research could provide scientific references for the rational development and utilization of land resources, protection and construction of ecological environment in Red River basin.