Background:Brucellosis is a worldwide zoonotic disease caused by Brucella spp.Brucella invades the body through the skin mucosa,digestive tract,and respiratory tract.However,only a few studies on human spontaneous abortion attributable to Brucella have been reported.In this work,the patient living in Shanxi Province in China who had suffered a spontaneous abortion was underwent pathogen detection and Brucella melitensis biovar 3 was identified.Case presentation:The patient in this study was 22 years old.On July 16,2015,she was admitted to Shanxi Grand Hospital,Shanxi Province,China because of one day of vaginal bleeding and three days of abdominal distension accompanied by fever after five months of amenorrhea.A serum tube agglutination test for brucellosis and blood culture were positive.At the time of discharge,she was prescribed oral doxycycline(100 mg/dose,twice a day)and rifampicin(600 mg/dose,once daily)for 6 weeks as recommended by the World Health Organization(WHO).No recurrence was observed during the six months of follow-up after the cessation of antibiotic treatment.Conclusions:This is the first reported case of miscarriage resulting from Brucella melitensis biovar 3 isolated from a pregnant woman who was infected through unpasteurized milk in China.Brucellosis infection was overlooked in the Maternity Hospital because of physician unawareness.Early recognition and prompt treatment of brucellosis infection are crucial for a successful outcome in pregnancy.
Background:Brucellosis was a common human and livestock disease caused by Brucella strains,the category B priority pathogens by the US Center for Disease Control(CDC).Identified as a priority disease in human and livestock populations,the increasing incidence in recent years in China needs urgent control measures for this disease but the molecular background important for monitoring the epidemiology of Brucella strains at the national level is still lacking.Methods:A total of 600 Brucella isolates collected during 60 years(from 1953 to 2013)in China were genotyped by multiple locus variable-number tandem repeat analysis(MLVA)and the variation degree of MLVA11 loci was calculated by the Hunter Gaston Diversity Index(HGDI)values.The charts and map were processed by Excel 2013,and cluster analysis and epidemiological distribution was performed using BioNumerics(version 5.1).Results:The 600 representative Brucella isolates fell into 104 genotypes with 58 singleton genotypes by the MLVA11 assay,including B.melitensis biovars 2 and 3(five main genotypes),B.abortus biovars 1 and 3(two main genotypes),B.suis biovars 1 and 3(three main genotypes),and B.canis(two main genotypes)respectively.While most B.suis biovar 1 and biovar 3 were respectively found in northern provinces and southern provinces,B.melitensis and B.abortus strains were dominant in China.Canine Brucellosis was only found in animals without any human cases reported.Eight Brucellosis epidemic peaks emerged during the 60 years between 1953 and 2013:1955-1959,1962-1969,1971-1975,1977-1983,1985-1989,1992-1997,2000-2008 and 2010-2013 in China.Conclusions:Brucellosis has its unique molecular epidemiological patterns with specific spatial and temporal distribution according to MLVA.Trial registration:IDOP-D-16-00101.
