[Objective] The aim was to investigate effect of intercropping and nitrogen regulation on nitrate and apparent loss of nitrogen, providing theoretical references for reasonable fertilization and pollution reduction. [Method] In the research, deeprooted eggplants and shallow-rooted scallions were intercropped with nitrogen regulat- ed to study on effect of the cropping system on temporal and spatial changes of ni- trate nitrogen and apparent loss of nitrogen. [Result] When the fertilizers were re- duced, contents of nitrate were less in intercropped field than that in mono-cropped one, indicating that eluviation of nitrate would be blocked and nitrate accumulation would be lowered effectively by intercropped eggplants and scallions. With intercrop- ping adopted, the reduced fertilizers would lower nitrate content in soil, deep soil in particular, and nitrate was significantly lower than that fertilized with conventional quantity during vegetable growth period. In addition, nitrate accumulation in soil profiles was also significantly shorter in field applied with reduced fertilizers than that with conventional one. It was shown that intercropping and fertilizer reduction would both cut apparent loss of nitrate down in varying degrees. [Conclusion] The research lays basis for high production of vegetables, reasonable fertilization and pollution reduction.