[Objective] The research aimed to investigate the bioturbation effects of Branchiura sowerbyi (Tubificidae) on the vertical transport of sedimentary particles in paddy field,and explore the bioturbation effects and mechanism of benthic Annelida in coupling process of benthic-pelagic interface.[Method]Using chemically stable glass beads as tracers,the vertical transport of sedimentary particles in paddy field was analyzed comparatively with and without B.sowerbyi.[Result]After 10 days' bioturbation of B.sowerbyi,41.3% of the glass beads on the surface of sedimentary particles were transferred downward to the maximum depth of 9.4 cm,and the vertical transportation rate of sedimentary particles was 1.370×10-3/(g·cm2·d). 25.8% and 17.3% of glass beads at a depth of 6 cm were transferred upwards and downwards respectively after bioturbation,to the maximum depth of 5.2 and 2.7 cm respectively,and the vertical transportation rates of sedimentary particles were 8.557×10-4 and 5.738×10-4/(g·cm2·d) respectively.[Conclusion]The sedimentary particles on the surface and deep layer of the paddy field were vertically shifted by the physical activities of B.sowerbyi,thus changed the sedimentary environment.