To investigate the genetic basis of drought tolerance in soybean(Glycine max L.Merr.) a recombinant inbred population with 184 F2:7:11 lines developed from a cross between Kefeng1(drought tolerant) and Nannong1138-2(drought sensitive) were tested under water-stressed and well-watered conditions in field and greenhouse trials.Traits measured included leaf wilting coefficient,excised leaf water loss and relative water content as indicators of plant water status and seed yield.A total of 40 quantitative trait loci(QTLs) were identified:17 for leaf water status traits under drought stress and 23 for seed yield under well-watered and drought-stressed conditions in both field and greenhouse trials.Two seed yield QTLs were detected under both well-watered and drought-stressed conditions in the field on molecular linkage group H and D1b,while two seed yield QTLs on molecular linkage group C2 were found under greenhouse conditions.Several QTLs for traits associated with plant water status were identified in both field and greenhouse trials,including two leaf wilting coefficient QTLs on molecular linkage group A2 and one excised leaf water loss QTL on molecular linkage group H.Phenotypic correlations of traits suggested several QTLs had pleiotropic or location-linked associations.These results will help to elucidate the genetic basis of drought tolerance in soybean,and could be incorporated into a marker-assisted selection breeding program to develop high-yielding soybean cultivars with improved tolerance to drought stress.
Drought stress has long been a major constraint in maintaining yield stability of soybean(Glycine max(L.) Merr.) in rainfed ecosystems.The identification of consistent quantitative trait loci(QTL) involving seed yield per plant(YP) and drought susceptibility index(DSI) in a population across different environments would therefore be important in molecular marker-assisted breeding of soybean cultivars suitable for rainfed regions.The YP of a recombinant line population of 184 F2:7:11 lines from a cross of Kefeng1 and Nan-nong1138-2 was studied under water-stressed(WS) and well-watered(WW) conditions in field(F) and greenhouse(G) trials,and DSI for yield was calculated in two trials.Nineteen QTLs associated with YP-WS and YP-WW,and 10 QTLs associated with DSI,were identified.Comparison of these QTL locations with previous findings showed that the majority of these regions control one or more traits related to yield and other agronomic traits.One QTL on molecular linkage group(MLG) K for YP-F,and two QTLs on MLG C2 for YP-G,remained constant across different water regimes.The regions on MLG C2 for YP-WW-F and MLG H for YP-WS-F had a pleiotropic effect on DSI-F,and MLG A1 for YP-WS-G had a pleiotropic effect on DSIG.The identification of consistent QTLs for YP and DSI across different environments will significantly improve the efficiency of selecting for drought tolerance in soybean.
