A computer program for small angle X-ray scattering （SAXS） data processing and analysis named S.exe written in Intel Visual Fortran has been developed. This paper briefly introduces its main theory and function.
In this paper, we present a novel design scheme of temperature-jump(T-jump) area for microfluidic device.Numerical simulation and experimental research of thermal characteristics of the solution in microchannels is completed.Numerical simulation of the temperature-jump microchannel is analyzed to study the heat transfer characteristics by comparing performance of three proposed configurations.Calculation of the power requirement is discussed in the dimensional design of microheater. Temperature-sensitive fluorescent dye is applied to investigate the temperature field of microchannel heated by a designed microheater. It is found that the T-jump microfluidic device can provide rapid heating for solutions with strong convection heat transfer ability.
Elucidating the structure of large biomolecules such as multi-domain proteins or protein complexes is challenging due to their high flexibility in solution. Recently, an ＂integrative structural biology＂ approach has been proposed, which aims to determine the protein structure and characterize protein flexibility by combining complementary high- and lowresolution experimental data using computer simulations. Small-angle x-ray scattering（SAXS） is an efficient technique that can yield low-resolution structural information, including protein size and shape. Here, we review computational methods that integrate SAXS with other experimental datasets for structural modeling. Finally, we provide a case study of determination of the structure of a protein complex formed between the tandem SH3 domains in c-Cb1-associated protein and the proline-rich loop in human vinculin.
