在利用ISSR技术对黄藤进行分子生物学研究过程中,对影响PCR扩增效果的一些因素,如模板DNA浓度、Mg2+浓度、dNTPs浓度、Taq DNA聚合酶浓度、引物浓度以及退火温度等指标进行筛选和优化,获得用于黄藤ISSR-PCR分析最适宜的PCR反应条件:20μLPCR反应体积中含有1×缓冲液,20 ng模板DNA,2 mmol.L-1MgC l2,100μmol.L-1dNTP,1.5 U TaqDNA聚合酶,0.4μmol.L-1引物,2%甲酰胺,0.5 mmol.L-1亚精胺.扩增程序为:94℃预变性5 m in;再35个循环:94℃30 s,52℃45 s和72℃1.5 m in;最后72℃延伸7 m in.
This paper dealed with the differences between albino and normal seedlings of Calamus nambarensis var.xishuangbanensis in terms of growth and photosynthesis.Thirteen families among 22 families were found to have one to three albinos,the percent of which varying from 0.4 to 2.2 percents,while the percent of albinos among all 22 families was about 0.7 percent.The number of albino leaves and the length of longest leaves were 1.9±0.3 and 22.32±3.49 cm respectively, which were less than that of 2.0±0.0 and 26.09±3.26 cm of normal seedlings,accounting for 95% and 85% of normal seedlings.The albinos hadn’t chlorophyll a,but some chlorophyll b.The total content of chlorophyll was 0.02 mg·g-1FW, much less than that of 8.15 mg·g-1FW of normal seedlings, accounting for 0.2 percent.Whether photosynthesis active radiation（PAR） was 800 μmol·m-2·s-1 or （1 600） μmol·m-2·s-1,the photosynthetic rate of albinos was less than that of normal seedlings,accounting for about 20 percents.The data of net photosynthetic rate of albino evaluatde by the authors were also
