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国家自然科学基金(30570170)

作品数:3 被引量:7H指数:2
相关作者:王钦美付杨敖曼高翔王丽更多>>
相关机构:东北师范大学更多>>
发文基金:国家自然科学基金长春市科技发展计划项目更多>>
相关领域:生物学更多>>

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Volatile Compounds in the Flowers of Freesia Parental Species and Hybrids被引量:4
2007年
For centuries, freesia has been one of the most important crops in the floriculture industry. Here, aqua-space samples collected from entire flowers of diploid Freesia refracta, three tetrapioid freesia cuitivars, and interspecific hybrids of three tetraploid freesia cultivars were analyzed using gas chromatograph coupled with mass selective detector, in all, 75 different compounds were identified. The compounds were mainly terpenes, hydrocarbons, alcohols, fatty acid esters and aromatic class compounds. Among these, iinalool was detected from all the sweet-scented flowers except for scentless white tetraploid F. hybrida. Stable inheritance of linalool between F. hybrida and their Ft progeny was observed. Based on the present analyses, the relationship between the aroma of freesia and iinalooi was discussed.
Yang Fu Xiang Gao Yiqun Xue Yuejun Hui Fengqing Chen Quanping Su Li Wang
香雪兰花瓣总RNA的提取和cDNA文库的构建被引量:3
2008年
以不同开放时间的红色香雪兰的花瓣为材料,利用CTAB裂解、LiCl沉淀的方法提取出高质量的总RNA,经纯化mRNA后,合成双链cDNA,加上EcoRⅠ和XhoⅠ接头,用胶回收的方法将500 bp以上的cDNA回收并连在载体上,获得了重组率为89%,滴度为4.8×105pfu/mL的香雪兰花瓣质粒cDNA文库,为研究香雪兰花香和花色的相关基因奠定了基础.
付杨高翔敖曼王钦美王丽
关键词:香雪兰总RNA提取CDNA文库
Specific Proteins in the Indirect Somatic Embryogenesis of Freesia Refracta
2007年
Using the young inflorescence segments of Freesia refracta as explants, indirect somatic embryogenesis of somatic cells was induced in a N6 medium supplemented with some exogenous hormones. SDS-polyacrylamide gel electrophoresis(SDS-PAGE) was used for the analysis of soluble proteins produced during the somatic embryogenesis of this plant. There are six polypeptides, which might play some roles in the process of somatic embryo development. Tltree polypeptides(45, 53 and 55 kD) were detected in the stages of embryogenic callus, globular embryoid, and embryoid with coleoptiles, except the embryoid with leaf. One polypeptide( 83 kD) was specific for the stages of embryoid with eoleoptiles and embryoid with leaf. One polypeptide(37 kD) was detected in the first two stages, namely, embryogenic callus and globular embryoid. One polypeptide(35 kD) was regularly synthesized in each stage, from embryogenic callus to embryoid with leaf.
GAO XiangHUI Yue-junXUE Yi-qunFU YangAO ManWANG Li
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