A rapid and concentration-dependent generation of superoxide anion (·O-2), measured with a superoxide-specific Cypridina luciferin-derived chemiluminescent reagent, was observed when two lanthanide salts (LaCl3 and GdCl3) were added to tobacco (Nicotiana tabacum) cell suspension culture. Addition of superoxide dismutase (480 U·ml -1 ) and Tiron (5 μmol·L -1 ) to cell culture suspension decreases the level of lanthanide cation-induced ·O-2 generation, suggesting that ·O-2 generation is extra-cellular. Pretreatment of the cell culture suspension with diphenyleneiodonium (10 and 50 μmol·L -1 ), quinacrine (1 and 5 mmol·L -1 ) and imidazol (10 mmol·L -1 ), inhibitors of NADPH oxidase, notably inhibits the generation of superoxide induced by lanthanide cation, implying the possible involvement of activation of NADPH oxidase. In addition, addition of SHAM (1 and 5 mmol·L -1 ), azide (0.2 and 1 mmol·L -1 ), inhibitor of peroxidase, has no influence on ·O-2 generation.