A method of DNA probes immobilization and specific target sequences capture in a microfluidic chip was presented.Acrydite-modified DNA probes are immobilized in silanized glass microfluidic channels ]via] photopolymerization in a polyacrylamide matrix.The resulting polymeric,hydrogel plugs are porous under electrophoretic conditions,and the immobilized DNA probes can be hybridized with fluorescence labeled complementary DNA.The total analysis process can be completed within 5 min,and the limit of detection is 0.1 μmol/L.This method is simple,rapid and feasible.The double-stranded DNA can be chemically denatured,and the chip is reusable.The conditions for photopolymerization,hybridization,and denaturation were discussed as well.
Hydrogel micropatterns of poly(ethylene glycol) and polyacrylamide were prepared with a facile photolithographic method. Monomer solutions containing photoinitiator were directly polymerized by UV illumination through a transparency photomask, forming 2-dimensional gel patterns on silanized glass surfaces. The chemically patterned surfaces thus prepared could be used as the template for patterning mammalian cells and formation of structured droplets.
In this paper, we report an integrated microfluidic chip for drug-metabolism. The chip contains three layers: the top PDMS cover, the middle quartz slide with microchannels and microwells, the bottom PDMS layer for cell culture. Microsomes isolated from human liver were encapsulated in microwells by sol-gel method.Using this design, the determination of the metabolites and drug cytotoxicity assessment can be carried out within a single microfluidic device, providing an alternative technique for the high-throughput parallel drug screening and drug interactions in the metabolic pathways.
