[ Objective] To discuss regional response of reference evapotranspiration ( ETo ) to global climate change and its influencing factors. [Method] Penman-Monteith equation was adopted to calculate ET0 in Australia from 1998 to 2007. Spatiotemporal change characteristics were analyzed by using GIS spatial analysis tools and relationships between ETo and main climate factors were also analyzed. [ Resultsl The results showed that multi-year average ET0 increased from the east and south part to the northwest part and inland, and its distribution was consistent with climate zones. Multi-year average ETo of the whole region was 1 750 mm, obtaining minimum and maximum values in 2000 and 2002 respectively. Regional ETo decreased in the order of summer, spring, autumn and winter; January and December got the highest monthly ET0, while June got the lowest value 79.55 mm. ETo positively correlated with mean temperature and solar radiation, R2 for each were 0.83 and 0.94, while the relationship between ETo and average relative humidity was negative, and precipitation had no significant relationship with ET0. [ Conclusion] This research could provide important reference for crop water requirement study and making irrigation method for Australia.