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国家自然科学基金(31171485)

作品数:7 被引量:35H指数:3
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超高效液相色谱法分析稻米酚酸化合物组分及其含量被引量:16
2015年
【目的】稻米酚酸化合物是天然抗氧化物的重要来源,本研究建立糙米和精米中酚酸化合物的超高效液相色谱的定性定量方法,并分析其在籽粒中的分布特征。【方法】以没食子酸、原儿茶酸、龙胆酸、对羟基苯甲酸、绿原酸、香草酸、咖啡酸、丁香酸、对香豆酸、阿魏酸、芥子酸、异阿魏酸、2-羟基肉桂酸和反式肉桂酸14种酚酸化合物标准品,对糙米和精米中酚酸进行定性和定量分析。色谱柱为Agilent ZORBAX Eclipse Plus C18(2.1 mm×50 mm,1.8μm-Micron),流动相为0.1%乙酸水溶液(A)/乙腈(B),柱温30℃,流速为0.5 m L·min-1,采用梯度洗脱,洗脱程序为0—1 min,8%—10%B;1—2.5 min,10%—13%B;2.5—5.5 min,13%B;5.5—6 min,13%—21%B;6—6.5 min,21%—27%B;6.5—7.5 min,27%—50%B;7.5—9 min,50%—100%B;9—12 min,100%B;12—12.5 min,100%—8%B。检测波长为280和325 nm。【结果】14种酚酸在8 min内完全分离,经质谱鉴定,精米及糙米中检测出的物质共有11种,分别是没食子酸、龙胆酸、对羟基苯甲酸、香草酸、咖啡酸、丁香酸、对香豆酸、阿魏酸、芥子酸、异阿魏酸和反式肉桂酸。可进行定量分析的酚酸共8种,分别是对羟基苯甲酸、香草酸、丁香酸、对香豆酸、阿魏酸、芥子酸、异阿魏酸和反式肉桂酸,线性范围为5—220μg·m L-1(R2=0.9994—0.9999),检出限为0.002—0.03μg·m L-1,定量限为0.004—0.08μg·m L-1,回收率为84.11%—114.43%。对羟基苯甲酸、香草酸、丁香酸、对香豆酸、阿魏酸、芥子酸、异阿魏酸和反式肉桂酸在精米和糙米中的含量差异显著,其中对羟基苯甲酸、香草酸、丁香酸、对香豆酸、阿魏酸、芥子酸和反式肉桂酸集中存在于胚乳外层中,精米/糙米含量百分比变幅为4.52%—16.73%。而异阿魏酸在稻米籽粒中分布较均匀,其精米/糙米含量百分比达到45.86%。【结论】该方法简便、快速、准确、可靠,不仅适合稻米中酚酸化�
张娜王国祥Abacar Jose Daniel刘正辉丁承强唐设李刚华王绍华丁艳锋
关键词:酚酸超高效液相色谱糙米精米
高效液相体积排阻色谱法测定稻米支链淀粉链长的相对分子质量分布被引量:5
2013年
【目的】建立稻米支链淀粉链长相对分子质量分布的高效液相体积排阻色谱法(high performance sizeexclusion chromatography,HPSEC)的测定方法。【方法】先采用丁醇沉降法分离提纯稻米支链淀粉,纯化后的支链淀粉经异淀粉酶酶解切开分支糖苷键,然后运用HPSEC测定其相对分子质量分布。建立了稻米支链淀粉链长相对分子质量分布的HPSEC分析方法:Shodex SB-G、Shodex SB-803(1 000—100 000)、Shodex SB-802.5(300—10 000)3根凝胶色谱柱串连,柱温40℃;流动相为0.1 mol.L-1Tris+0.1 mol.L-1NaCl,pH=7.40,流速0.5 mL.min-1;采用示差折光检测器(Differential refractive index detector,RI),检测器温度为37℃;待测样品浓度为5mg.mL-1,进样量20μL。【结果】本法提纯的稻米支链淀粉:淀粉含量为99.4%、蛋白残余为0.02%、支链淀粉碘复合物最大吸收峰为517 nm,为高纯度支链淀粉。供试大米样品脱分支支链淀粉的平均聚合度(DP)分布在2—120,无杂峰干扰。【结论】试验结果和方法验证表明本方法数据可靠、重复性好、简单易行。
杨小雨刘正辉李刚华王强盛王绍华丁艳锋
关键词:相对分子质量分布
Effects of Nitrogen Fertilization and Genotype on Rice Grain Macronutrients and Micronutrients被引量:1
2014年
High nitrogen (N) input features China's intensive rice production system. To elucidate N and genotype effects on accumulation of macronutrients and micronutrients in grains of japonica rice, and to discuss its significance in rice production, a three-year field experiment involving six japonica rice varieties and seven N treatments were performed. Macronutrients (Ca, Mg, K, and Na) and micronutrients (Cu, Fe, Mn, and Zn) concentrations in brown and milled rice were measured using an atomic absorption spectrophotometer. For macronutrients, no consistently significant effect of N was detected in both brown and milled rice. For micronutrients, N showed significant effect, especially in lowering Zn accumulation in brown and milled rice. In addition, N tended to increase Fe concentration in milled rice. Genotype showed larger effect on distribution of minerals in milled rice than N. The high-yielding variety, Wuyunjing 7, accumulated larger proportion of Mg, K, and Zn in the milled rice as compared with the other five varieties and could be of value for rice breeding programs aiming at high nutritional quality. The results demonstrated differences in response to N between macronutrients and micronutrients, and are of significance for coping with 'hidden hunger' both in humans and crops through agronomical practices.
LIN Zhao-miaoNING Hui-fengBI Jun-guoQIAO Jiang-fangLIU Zheng-huiLI Gang-huaWANG Qiang-shengWANG Shao-huaDING Yan-feng
关键词:MACRONUTRIENTMICRONUTRIENTGENOTYPERICE
粳稻碾米品质的基因型差异及其与稻米理化特性的关系被引量:2
2013年
以177份武育粳3号突变体为材料,通过田间试验,研究了不同突变体材料的糙米率、精米率和整精米率等碾米品质指标的基因型差异,并分析了其碾米品质指标与粒形、垩白、直链淀粉含量、蛋白质含量等生化组分之间的关系。研究结果表明,突变体材料之间在碾米品质上呈现显著的基因型差异。糙米率分布区间为69.5%~86.2%,平均值为81.7%;精米率分布区间为52.0%~76.3%,平均值为71.4%;整精米率分布区间为29.4%~73.9%,平均值为68.3%。筛选到的整精米率较低和较高的20份突变体可为碾米品质形成机制研究提供新材料。相关分析结果表明,整精米率与垩白粒率呈极显著负相关(r=-0.291**),与粒长和粒重呈显著和极显著(r=0.209*,r=0.400**)正相关。整精米率与总蛋白、谷蛋白含量呈极显著负相关(r=-0.429**,r=-0.421**)。本研究结果初步表明了垩白粒率、粒形和蛋白质含量等理化指标与整精米率之间的关系,为揭示碾米品质形成的理化基础提供了参考。
衣政伟林赵淼刘正辉李刚华王绍华王强盛丁艳锋
关键词:粳稻碾米品质基因型
Analysis of Variations in White-Belly and White-Core Rice Kernels Within a Panicle and the Effect of Panicle Type
2014年
This study aims to investigate the variation in occurrence of white-belly rice kernel(WBRK) and white-core rice kernel(WCRK) among different positions within a panicle. Twenty-four M4 mutants involved in four panicle types, namely the compact, intermediate, loose, and chicken foot panicle were used. They derived from a japonica rice cultivar Wuyujing 3. Considerable differences in morphological characters existed among the four types of panicle, especially in panicle length, the secondary branch number and ratio of grain number to total branch length. Marked differences were found in WBRK and WCRK among different positions within a panicle for all types of panicle. In general, grains located on the primary rachis and top rachis branches had higher WBRK and WCRK percentage than those on the secondary rachis and bottom rachis branches. WCRK exhibited larger variation among grain positions than WBRK did. Moreover, there was a significant difference in WCRK/WBRK among grain positions within a panicle, with primary rachis and top rachis branches having higher values than the secondary and bottom rachis. In addition, panicle type showed no significant effect on the pattern of WBRK and WCRK occurrence within a panicle. The results indicated the difference in mechanism of WBRK and WCRK formation in grain position within a panicle, and are valuable for breeding and agronomic practices aimed at lowering chalky grain rate.
ZHANG Xin-chengMd A.AlimLIN Zhao-miaoLIU Zheng-huiLI Gang-huaWANG Qiang-shengWANG Shao-huaDING Yan-feng
越光/9311染色体片段置换系的构建及稻米黏滞性谱特征值QTL分析被引量:3
2015年
研究以籼稻品种9311为受体亲本,粳稻品种越光为供体亲本构建染色体片段置换系(CSSLs)并对RVA谱特征值进行QTL定位,为稻米蒸煮食味品质的遗传改良创造理想条件。研究利用612对SSR标记对亲本(越光和9311)进行多态性筛选,使用检测出的多态性引物对染色体片段置换系株系进行鉴定。共鉴定出138个株系,并构成染色体片段置换系。该染色体片段置换系的水稻全基因组覆盖率达到89%,置换系群体中超过80%的株系携带的供体亲本染色体片段数少于5个,其中单片段置换系为51个,比例为36.95%。利用该群体共定位到位于6条不同染色体上9个区域的10个QTL,表型贡献率范围为8.85%~42.65%,其中q BDV-1、q PT-4.1、q PT-4.2和q CSV-3等4个QTL为首次报道。q HPV-2位于第2染色体上标记RM341与RM525之间,q BDV-2位于第2染色体RM341与RM1385标记之间,与该染色体上支链淀粉合成有关基因等位。本研究构建的CSSLs及定位的控制RVA谱特征值相关QTL,为稻米蒸煮食味品质的研究创造了有利条件。
邵昕陈忠明刘正辉丁承强唐设李刚华王绍华丁艳锋
关键词:染色体片段置换系蒸煮食味品质RVA
Physicochemical and Sensory Properties of japonica Rice Varied with Production Areas in China被引量:8
2013年
Northeast of China and Jiangsu Province are major production areas of japonica rice in China.Rice from northeast of China is well-known for its good-eating and appearance quality,and that from Jiangsu Province is viewed as inferior.However,little is known concerning the difference in physicochemical and sensory properties of rice between the major two production areas.Analysis of 16 commercial rice samples showed marked differences in physicochemical properties,including chalky grain rate,contents of amylose and protein and pasting properties between the two main areas.Northeastern rice contained more shortchain amylopectin as compared with Jiangsu rice.However,Jiangsu rice is comparable to northeastern rice in terms of sensory quality including overall acceptability and textural properties of springiness,stickiness and hardness as evaluated by trained panel.Our results indicated the limitation of conventional index of physicochemical properties,and suggested the necessity of identification of new factors controlling rice sensory property.In addition,the taste analyzer from Japan demonstrates limitation in distinguishing the differences between northeastern and Jiangsu rice,and therefore needs localization to fit China.
YANG Xiao-yuLIN Zhao-miaoLIU Zheng-huiMd A AlimBI Jun-guoLI Gang-huaWANG Qiang-shengWANG Shao-huaDING Yan-feng
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