Vernalization,the process of a long exposure to low temperature to induce flowering in plants,is essential for plants to adapt to cold winters.The presence of vernalization genes Vrn-A1,-B1,and-D1 in four cornerstone breeding parents of wheat in China(Funo,Mentana,Yanda 1817,and Bima 4)and 322 derivative varieties(mostly winter wheat)from these parents were determined using PCR based molecular method.The frequencies of the VRN-1 genes in these derivative varieties were in order of Vrn-D1(67.1%)> Vrn-B1(19.6%)> Vrn-A1(5.3%),which are similar as the former conclusion that Vrn-D1 is associated with the latest heading time,Vrn-A1 the earliest,and Vrn-B1 intermediate values.The frequencies of Vrn-A1 and Vrn-B1 loci in the derivative varieties from winter wheat zones were higher than that from spring winter zones.Based on the wheat breeding history in China and the fact of non-random distribution of Vrn-A1 and Vrn-B1 in the derivative varieties from the four parents,there could be a strong selective effect on VRN-1 genes in different regions where the derivative varieties were cultivated.
Synthetic hexaploid wheat (Triticum turgidum × Aegilops tauschii) was created to explore for novel genes from T. turgidum and Ae. tauschii that can be used for common wheat improvement. In the present paper, research advances on the utilization of synthetic hexaploid wheat for wheat genetic improvement in China are reviewed. Over 200 synthetic hexaploid wheat (SHW) accessions from the International Maize and Wheat Improvement Centre (CIMMYT) were introduced into China since 1995. Four cultivars derived from these, Chuanmai 38, Chuanmai 42, Chuanmai 43 and Chuanmai 47, have been released in China. Of these, Chuanmai 42, with large kernels and resistance to stripe rust, had the highest average yield (> 6 t/ha) among all cultivars over two years in Sichuan provincial yield trials, outyielding the commercial check cultivar Chuanmai 107 by 22.7%. Meanwhile, by either artificial chromosome doubling via colchicine treatment or spontaneous chromosome doubling via a union of unreduced gametes (2n) from T. turgidum-Ae. tauschii hybrids, new SHW lines were produced in China. Mitotic-like meiosis might be the cytological mechanism of spontaneous chromosome doubling. SHW lines with genes for spontaneous chromosome doubling may be useful for producing new SHW-alien amphidiploids and double haploid in wheat genetic improvement.
Wuyun YangDengcai LiuJun LiLianquan ZhangHuiting WeiXiaorong HuYouliang ZhengZhouhu HeYuchun Zou
Synthetic hexaploid wheat(SHW) represents a valuable source of new resistances to a range of biotic and abiotic stresses.A recombinant inbred line(RIL) population with 127 recombinant inbred lines derived from a SHW-derived variety Chuanmai 42 crossing with a Chinese spring wheat variety Chuannong 16 was used to map QTLs for agronomic traits including grain yield,grains per square meter,thousand-kernel weight,spikes per square meter,grain number per spike,grains weight per spike,and biomass yield.The population was genotyped using 184 simple-sequence repeat(SSR) markers and 34 sequence-related amplified polymorphism(SRAP) markers.Of 76 QTLs(LOD>2.5) identified,42 were found to have a positive effect from Chuanmai 42.The QTL QGy.saas-4D.2 associated with grain yield on chromosome 4D was detected in four of the six environments and the combined analysis,and the mean yield,across six environments,of individuals carrying the Chuanmai 42 allele at this locus was 8.9% higher than that of those lines carrying the Chuannong 16 allele.Seven clusters of the yield-coincident QTLs were detected on 1A,4A,3B,5B,4D,and 7D.
To determine the effect of 6 + 8 and 1.5 + 10 HMW-GS of synthetic hexaploid wheat (SHW) on main quality parameters of wheat, a set of recombinant inbred lines (RILs) derived from the cross between a SHW with N, 6 + 8, 1.5 + 10 HMW-GS and a cultivar Chuanyu 12-1 (CY12-1) with 1, 7 + 8, 2 + 12 were planted in three environments in 2005 and 2006 and totally 16 quality parameters were tested for each line. Significant differences in all tested quality parameters but flour yield were observed between the two parents. The mean values of the RILs were intermediate to the parents for grain and protein parameters and some farinograph parameters, flour water absorption (FWA), and farinograph softening (SOF) but beyond parents at dough stability time (DST), breakdown time (BRT), quality number (QN), noodle score (NS), and loaf volume (LOV). All of the quality traits, especially in grain hardness (GH), zeleny sedimentation volume (SED), and most of farinograph parameters had significant difference between the different HMW-GS components. The effects of different alleles of HMW-GS at same locus (Glu-A1 or Glu-B1 or Glu-D1) on the different quality parameters were also different and affected by the other two loci. For most of parameters tested, 6 + 8 was better than 7 + 8 and there was no difference between 1.5 + 10 and 2 + 12. End-use quality was greatly influenced by components of HMW-GS. The components of 1, 6 + 8, 1.5 + 10 had the highest LOV and bread score (BS) values, whereas the components of 1, 7 + 8 and 1.5 + 10 had the highest NS values. Noodle score performed a positive linear relationship with falling number (FN) and its relationships to other quality parameters were affected by environments. Loaf volume had a significant negative relationship to SOF and positive associations with most of quality parameters. It could be concluded that HMW-GS 6 + 8 from SHW had better overall quality characteristics than 7 + 8, whereas the effects of 1.5 + 10 on quality was different in respect to quality parameters and the HMW-GS
