[Objective] The paper was to study the karyotype of Chi-lin fish （Varicorhi- nus macrolepis） from Taishan mountain. [Method] The chromosome number and karyotype of an endemic Chi-lin fish were studied using the head kidney cells. [Re- sult] The diploid chromosome number （2n） of Chi-lin fish was 50, and the karyotype of V. macrolepis was 2n=50,16m＋14sm＋20t, NF =80. No sex-chromosome was found in Chi-lin fish. [Conclusion] A detailed karyotype of this endemic cyprinid fish of Taishan Mountain was established for the first time in this study.
[Objective] A mobility measurement and a regeneration assay were conducted to explore the behavioral effects of Cu2＋ on planarian Dugesia japonica. [Method] Dugesia japonica was respectively treated with different concentrations of copper solution for 4 and 6 d, and the blank control group was set. The mobility measurement and a regeneration assay of copper ions were determined by mIC50 and rIC50 . [Result] The mobility IC 50 for adult D. japonica at 24, 48, 72 and 96 h were 86, 3.73, 3.47 and 2.61 mg Cu2＋ /L, respectively. The mobility IC50s for newborns was 2.22, 1.64, 0.87 and 0.93 mg Cu2＋ /L, respectively. Although there was no significant difference between 24 and 48 h, and between 72 and 96 h of copper exposure, Cu2＋ decreased newborns locomotor behavior in a concentration-dependent manner at sub-toxic concentrations. The regeneration IC50 of eyespots and auricles for adult D. japonica at 6 d were calculated as 0.76, 0.78 mg Cu2＋ /L, respectively. The number of eyespots and auricles regeneration in planarian D. japonica showed a concentration-dependent manner. Results showed that newborns mobility and adult regeneration assay for D. japonica in a concentration-dependent manner. [Conclusion] The results of the present study suggest that planarians can be regarded as a useful bioindicator species for toxicological studies in the future.
本试验旨在探讨环境内分泌干扰物双酚A(BPA)对涡虫抗氧化酶(SOD,CAT,GPx)的活性及相关基因表达的影响。采用不同浓度的BPA处理涡虫48 h,然后测定抗氧化酶活性,并应用半定量RT-PCR技术探讨相关基因的变化。在BPA胁迫下,SOD活性呈现上升趋势、CAT活性表现出了先上升后下降的趋势,GPx活性总体上变化不大。随BPA浓度的增加,Dj SOD m RNA表达量上升,并呈现剂量-反应关系,Dj CAT及Dj GPx m RNA表达量也呈现出明显的上调现象,但变化趋势并不同于酶活性。BPA诱导涡虫的氧化应激反应,改变抗氧化酶SOD、CAT基因的转录水平。
Placenta specific protein 8 （PLAC 8） also called onzin or C15, is a cys- teine-rich protein. It is widely distributed in eukaryotes, and its function has been extensively studied. It not only regulates cell division, differentiation, and apoptosis, but also plays an important role in the immune process. This article describes the latest progress of plac8 gene structure characteristics, evolution, expression and function and possible mechanisms.
