The qualitative and quantitative research on glycolipids compounds was analyzed by means of the flight time ultra-high performance liquid chromatography tandem mass spectrometry(UPLC-Q-TOF-MS) technology.The mass spectrometry was performed in reflective time-of-flight using electron spraying ionization.The components in monoglycosyldiglycerides(MGDG) and diglycosyldiglycerides(DGDG) were determined in positive ion mode,and the sulfoquinovosyl diacylglycerol(SQDG) were analyzed in negative ion mode.The results showed that each component in golycolipids can be separated effectively with gradient elution in the BEH C18 column.The proportion of each components accounts for total glycolipids can be determined by using degree elution.
The mass spectrometric fragmentation of TMS derivatives of sterols was studied and analyzed based on the mass spectrometry of the TMS derivatives of standards.The double bond numbers and the degree of the methylize,alkylize and the related position in the sterol nucleus and the side chains were confirmed by the characteristic ions.Total lipids were extracted using Bligh-Dyer method.Sterols were isolated by solvent partition with chloroform-hexane(1 ∶4,by volume),derivated by BSTFA,and analyzed by GC-MS.The regular pattern was used for identifying the sterols in a dinoflagellate from the Symbodinium sp.
